Gene Insertion in Caenorhabditis elegans to Compare Methods of Genome Transformation

by Elizabeth Ahrendsen
Faculty advisor: Faculty Sponsor: Dr. James Hampton

Previous methods used to insert genetic information into the genome of the nematode, Caenorhabditis elegans, have experienced numerous complications; therefore, we have begun to experiment with a new method. The purpose of our research is to rescue the knockout gene unc-119 in the C.elegans gamete using an Aerosol Beam Injector, microparticle bombardment, and a Particle Infusion Gun (PIG).

A definitive protocol has yet to be determined for the novel technique of Aerosol Beam Injection. This method is much like microparticle bombardment; however, an aerosolized form of DNA is used as a vector instead of gold-particles. Multiple parameters will be varied in these tests to determine the optimal settings for the machine as well as the preparation of the samples.